keratin1  (Boster Bio)

Journal: Skin Research and Technology

Article Title: Rutin alleviates psoriasis‐related inflammation in keratinocytes by regulating the JAK2/STAT3 signaling

doi: 10.1111/srt.70011

Figure Lengend Snippet: M5‐induced abnormal differentiation of HaCaT cells was ameliorated following Rutin treatment. (A–C) Western blotting assessed the protein levels of Keratin1 and Keratin5 in M5‐stimulated HaCaT cells and Rutin‐treated M5‐stimulated HaCaT cells. *** p < 0.001 vs. control; ## p < 0.01 and ### p < 0.001 vs. M5. Differential expression testing was performed with ANOVA.

Article Snippet: Subsequently, the membrane underwent blocking, followed by detection with specific primary antibodies, including Keratin5 (Cat#GTX11321, 1:1000, GeneTex, Irvine, CA, USA), Keratin1 (Cat#M01639‐1, 1:5000, BosterBio, Wuhan, China), p‐Janus kinase 2 (JAK2) (Cat#GTX132784, 1:1000, GeneTex), p‐signal transducer and activator of transcription 3 (STAT3) (Cat#bs‐55208R, 1:1000, Bioss, Beijing, China), and β‐actin (Cat#ab8226, 1:10 000, Abcam, Cambridge, Massachusetts, USA).

Techniques: Western Blot, Control, Quantitative Proteomics

Journal: PLoS ONE

Article Title: Epidermal Transglutaminase (TGase 3) Is Required for Proper Hair Development, but Not the Formation of the Epidermal Barrier

doi: 10.1371/journal.pone.0034252

Figure Lengend Snippet: The rate of wound closure in TGM3 −/− mice was not different compared to control animals (A) (n = 9), despite an increase in TGase 3 expression in healing epidermis (B). At day 9 post wounding the thickness of the epidermal layer in the wounded region was greater in wild type skin (C, E) when compared to mutant skin (D, F). Staining of healing skin with keratinocyte differentiation markers in wild type (G, J, M) and TGM3 −/− (H, I, K, L, N, O) animals demonstrated that while there was no change in the expression pattern of the keratinocyte markers loricrin (G–I) or keratin10 (J–L) between the mouse lines, keratin14 (M–O) already showed a more restricted staining pattern reminiscent of unwounded skin. (scale bars C, D 400 µm, E–G 100 µm)

Article Snippet: After blocking, sections were incubated for 1 h at RT with rabbit polyclonal antibodies against loricrin, involucrin, filaggrin, keratin1, keratin10, keratin14 (Covance Research Products), and trichohyalin (a kind gift from Dr. Edward O'Keefe, University of North Carolina, USA), mouse monoclonal antibodies against TGase 1 (Paesel+Lorei) or rat monoclonal antibodies against TGase 2 (Neomarkers) and TGase 3 (a kind gift from Dr. Kiyotaka Hitomi, Nagoya University, Japan).

Techniques: Control, Expressing, Mutagenesis, Staining

Journal: PLoS ONE

Article Title: Epidermal Transglutaminase (TGase 3) Is Required for Proper Hair Development, but Not the Formation of the Epidermal Barrier

doi: 10.1371/journal.pone.0034252

Figure Lengend Snippet: Proteins were extracted from wild type and TGM3 −/− hair with 2% SDS and 5% β-mercaptoethanol over night prior to SDS-PAGE separation and transferred to nitrocellulose. Membranes were probed with polyclonal antibodies against involucrin, trichohyalin, keratin10 and keratin14. Two prominent bands observed in the Coomassie stained SDS-PAGE of TGM3 −/− protein lysates were identified by tryptic peptide mass fingerprinting as keratin6hf (A) and keratin17 (B).

Article Snippet: After blocking, sections were incubated for 1 h at RT with rabbit polyclonal antibodies against loricrin, involucrin, filaggrin, keratin1, keratin10, keratin14 (Covance Research Products), and trichohyalin (a kind gift from Dr. Edward O'Keefe, University of North Carolina, USA), mouse monoclonal antibodies against TGase 1 (Paesel+Lorei) or rat monoclonal antibodies against TGase 2 (Neomarkers) and TGase 3 (a kind gift from Dr. Kiyotaka Hitomi, Nagoya University, Japan).

Techniques: SDS Page, Staining, Peptide Mass Fingerprinting